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Creation 39(4):20–23, October 2017

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Bioluminescence—the light of living things

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squid

Many creatures make their own light from a chemical process. Called bioluminescence, it occurs in a variety of organisms: insects, centipedes and millipedes, a snail, earthworms, echinoderms, fungi, fish, squid, as well as some microbes.

The mechanism

Bioluminescence requires a light-emitting pigment, known as a luciferin; the chemical reaction that turns energy into light is aided by an enzyme called luciferase (Latin lucifer, ‘light-bearer’). It is sometimes called ‘cold light’, because the efficiency with which this process turns chemical energy into light rather than wasting it as heat is extremely high; around 40%,1 some 20 times higher than an incandescent light bulb, and higher than the best fluorescent and LED bulbs.

Marine organisms alone exhibit more than four types of luciferin. Many of these creatures emit blue light which travels farther in water than the green light of fireflies, for example. The enzyme involved varies in structure between species even within the same phylum, and the variants show little correspondence with one another.2 This lack of similarity makes it impossible to establish a plausible common evolutionary origin for bioluminescence. Bioluminescence is therefore said to have arisen at least 40–50 times in evolutionary history.2 

©Paul H Yanceydragonfish
Stoplight loosejaws are small, deep-sea dragonfishes that have two types of light organs that glow either blue-green or red. To produce red light, their bioluminescent light excites a light-sensitive protein that in turn emits red light. The light then passes through a brown filter, making it appear even redder. These fish are among the few that can also see red light in the ocean.

Some bioluminescent creatures cannot produce their own light, but rely on others to do the job; several genera of squid use bacteria to produce their light, in a mutually beneficial co-living arrangement (symbiosis). The squid somehow monitors the performance of the bacteria, and strains that fail to produce enough light are rejected by a yet unknown mechanism. In some of these squid, the presence of bacteria causes the development of the light-producing organ.

The rest of the squid genera, though, make their own light, using a luciferin along with their individual luciferase enzyme.2 This divergence within one group defies easy evolutionary explanations.

Some ocean predators (e.g. certain fish) are bioluminescent, but are unable to produce a luciferin themselves. They have all the needed organs and enzymes, but have to get the luciferin by feeding on marine organisms that do have it—which are often not themselves able to use or produce light!

Purposes

Bioluminescence has many uses: mating, distracting prey or predators, luring prey, and communication. In some cases the use is unknown.

Firefly / Martin TampierBioluminscence

Evolutionists must assume that all features they observe must serve (or have served) some survival purpose. So bioluminescence in some mushrooms is a mystery. Only three fungal lineages glow (Omphalotus, Armillaria and Mycenoid species), and the intensity of the light they give out varies between species.3 Although some mushrooms apparently attract insects with their glow in order to spread their spores,4 this does not seem to be so in others. In species where the fibrous, nutrient-gathering mycelium (not the spore-bearing parts) glows, insects might even do more harm than good by eating these parts.3 Of course, God may have put features into creation with no other purpose than being ornamental, or showcasing His creativity.5 

Interestingly, some creatures use bioluminescence for counter-illumination. To disguise their shadow against light coming from above, some marine organisms use bioluminescence on their bellies to continuously match the ever-changing light intensity and colour. Evolutionists have not explained how such complex features could have developed gradually. An incomplete, poorly controlled predecessor of the mechanism would have provided little protection against predators. It would likely even have caused easier detection (e.g. when glowing at night before the organism developed a mechanism to reduce light emission effectively, or glowing the wrong colour, thus rendering the organism more visible).

Spider and firefly / Martin Tampierspider-catching-firefly

The best-known bioluminescent animals are fireflies or lightning bugs (Lampyridae). These beetles use specific light pulse patterns to communicate and locate their mates. The delay after the male’s last flash and the beginning of the female’s response is precisely timed, and is species-specific, so courtship is disrupted when this timing is off.6 Some female fireflies can imitate the patterns of other firefly species and will then attract males of these species—to prey on them!7 Sometimes, they will coordinate their light pulses while flying, leading to a spectacular display of precisely synchronized light flashes.

Lack of evolutionary explanations

The patterns of occurrence cause evolutionists to propose that bioluminescence evolved at least three times in fireflies. Supposedly, the larvae glowed first (as a deterrent to indicate bad taste and toxicity of the larvae) and the adults only retained the glow later. Subsequently, the glow transitioned from its function as a deterrent to its function in mating.6 But the development of communication codes, such as the male/female communication in fireflies or light-based communication in fish,8 seems impossible to explain by evolution. Both a code generation and interpretation system must exist simultaneously in the male and female in order to improve mating and so give a selective advantage.

fireflies
Male and female fireflies use different 'morse codes' to communicate

Evolutionists assume that it is ‘easy’ to evolve the capacity for bioluminescence, based on their assumption that the phenomenon has evolved in parallel so frequently. “The ingredients are usually not hard to come by. Stand in the dark, mix some egg white with oxygen and luciferin from, say, a jellyfish, and you’ll probably get a flicker of blue light,” suggests a recent article in National Geographic.9 

But the deeper question is, how did any such mechanism come about on its own? Bioluminescence involves both an enzyme and a luciferin, plus a control mechanism integrated with an organism’s nervous system, as well as genetic information to control both the development and the operation of light-emitting organs. Evolutionists have not plausibly explained how this extremely complex mechanism could emerge in a step-wise process, since each stage would have to be fully functional in order to be preserved by natural selection. Even relying on already-existing enzymes (the evolutionary origin of which is usually taken for granted) that then become part of some entirely new apparatus is a mind-boggling challenge for evolutionists to explain.

Conclusion

Because of their preexisting belief in the evolutionary story, evolutionists assert that bioluminescence came into existence independently dozens of times, over a long geological timeframe, in completely different phyla and under very different circumstances, yet leading to similar light-generation mechanisms, i.e. ‘convergence’. This is in effect an admission that it is not possible to reconstruct any coherent evolutionary history for it. And all explanations to date (including ones not discussed here) fall well short of a plausible account as to how such complex and varied mechanisms could have arisen through chance mutation and selection.

Rather, it is consistent with the evidence to hold that these features were specially created, part of the original design of many species—some of which may have subsequently lost such abilities. The astonishing breadth of variation within bioluminescence mechanisms is a testimony to the creativity of the Creator, and is intentional, rather than incidental.

Evolutionary mind-twisting

The most prominent hypothesis about how bioluminescence evolved supposes that after photosynthesis arrived and led to increasing amounts of oxygen in the atmosphere, the strongly antioxidant oxygenase-luciferin complex was a way to cope with this, and luminescence was merely incidental at first. Only after the ability to see also evolved, did other uses for the pigment become available.1

But organisms possess many other antioxidants, so why is a process that produces light at high energy expense still around today? Or how could organisms survive that did not have these very strong antioxidants? Moreover, experiments have shown that a rededication of an enzyme to another purpose by evolutionary processes is non-feasible.2

Even some evolutionists find problems with this explanation: “… for two groups, the fungi and the cypridinids, the oxygen requirements of their bioluminescent systems are known to be high, indicative of an origin at [oxygen] levels corresponding to present-day values, thus unlike the bacteria and fireflies. So we must conclude that if some luciferases originated as a mechanism for oxygen detoxification, the evidence indicates that this was not so for all.”3

The alternative proposed is that another type of enzyme, a ligase (which bonds two big molecules together), has evolved to become a luciferase.4 Yet even researchers supportive of the idea admit: “How the new oxygenase luminogenic function originated from AMP-ligases leading to luciferases is one of the most challenging mysteries of bioluminescence.”4

References and notes

  1. Ref. 2 of main text.
  2. Gauger AK, Axe DD (2011) The evolutionary accessibility of new enzyme functions: a case study from the biotin pathway. BIO-Complexity 2011(1):1–17.
  3. Wilson, T. and Hastings, J., Bioluminescence: Living Lights, Lights for Living, p. 132 Harvard College, 2013.
  4. Viviani, V.R., The origin, diversity, and structure function relationships of insect luciferases, Cellular and Molecular Life Sciences 59(11):1833–1850, November 2002.

References and notes

  1. Many earlier papers claimed c. 90% efficiency, but more recent research indicates it’s less than half that. See Ando, Y. et al., Firefly bioluminescence quantum yield and colour change by pH-sensitive green emission, Nature Photonics 2: 44–47, 2008 | doi:10.1038/nphoton.2007.251. See also comment in the same issue, Ugarova, N.N., Bioluminescence: Fireflies revisited, pp. 8–9 | doi:10.1038/nphoton.2007.259. Return to text.
  2. Haddock, Steven H.D. et al., Bioluminescence in the Sea, Annual Review of Marine Science 2:443–493, 2010 | doi: 10.1146/annurev-marine-120308-081028. Return to text.
  3. Out of the darkness, ABC Science, 16 January 2014,abc.net.au. Return to text.
  4. Glowing Mushrooms Use Bioluminescence to Attract Insects … , BioQuick News, March 20, 2015, bioquicknews.com. Return to text.
  5. Burgess, S., Added beauty of the peacock tail and the problems with the theory of sexual selection, J.Creation 15(2):94–102. Return to text.
  6. Branham, M.A. and Wenzel, J.W., The origin of photic behavior and the evolution of sexual communication in fireflies. Cladistics 19(1):1–22, 2003 | 10.1111/j.1096-0031.2003.tb00404.x. Return to text.
  7. Nguyen, T, Firefly's Flash Can Bring Sex or Death, livescience.com, 25 September 2007. Return to text.
  8. J.S. and Smith W.L., Repeated and Widespread Evolution of Bioluminescence Davis M.P., Sparks in Marine Fishes, PLoS ONE 11(6): e0155154 | doi:10.1371/journal.pone.0155154, 2016. Return to text.
  9. Judson, O., Luminous Life, nationalgeographic.com, 2015. Return to text.

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Readers’ comments

Ian H.
At a smaller end of the scale, living cells emit biophotons. Biophotons were discovered in 1922, when the Russian embryologist and histologist Alexander G.Gurwitsch (1874–1954) performed an experiment with onion roots. Only when after World War II photomultipliers became available to biomedical researchers, measurements proved the existence of cell radiation beyond doubt. They were able to detect a hundred times weaker photon streams and could even register single photons.
In the West, this happened first in 1954–55 when the Italian biophysicists L.Colli and U.Facchini and their collaborators at the University of Milan verified Gurwitsch’s discoveries with the new technology and showed that sprouts of various plants emit visible light. However, after their two publications they did not continue the investigations.
While Russian scientists had done extensive investigations with photomultipliers since the late 1940s, systematic Western research in this field only started with the experiments of the Australian physical chemist Terence I.Quickenden in the late 1960s and early 1970s, and finally with the work of the German biophysicist Fritz Albert Popp from 1974 onwards.
Popp and his collaborators at the University of Marburg, and later at the University of Kaiserslautern and the International Institute of Biophysics at Kaiserslautern and Neuss, were the first to carry out systematic experimental and theoretical investigations of all questions related to this new biological phenomenon.
All living organisms, including humans, emit a low-intensity glow that cannot be seen by the naked eye, but can be measured by photomultipliers that amplify the weak signals several million times and enable the researchers to register it in the form of a diagram.
Eric B.
Martin, an excellent article thank you, and Ian H comment info more than interesting.

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