This article is from
Journal of Creation 27(1):42–45, April 2013

Browse our latest digital issue Subscribe

Engineering without an engineer, or shedding new light on feats in the junkyard of DNA

A review of Evolution: A View from the 21st Century by James A. Shapiro
Financial Times Press Science, Upper Saddle River, NJ, 2011



This is a thought-provoking book by a highly eminent expert in microbiology and bacterial molecular genetics. He developed the concept of “natural genetic engineering” over the last 20 years, and produced numerous publications in the relevant scientific literature. Thus one may perhaps be a little taken aback considering the number of published commentaries that have been offered since the appearance of his book.

Bearing in mind a variety of reviews in the very recent literature and a range of accessible appraisals of Shapiro’s book (incl. a series of reports and exchanges from ID proponents since 20111), I would like to restrict my brief review to a few principal observations. In view of the slender size of Shapiro’s book (the actual length of main text consists only of 147 pages, including about 20 pages with tables), the length of all reviews taken together will soon match the extent of the original—this is also testament to the author’s standing and naturally the high profile debate on departures from Darwinian evolution.

Cover, captured

A striking photo of the wings of a mimetic moth from Costa Rica adorns the cover of Shapiro’s book and tenders a promising opening into an up-to-date scientific account of biology in general and evolution in particular. However, don’t be baffled that there is neither the expected traditional discourse of evolutionary biology nor a specific reference to moths in the book other than mentioning them in two passages in passing. It’s therefore all the more provocative considering that another moth—the peppered moth, Biston betularia—has been portrayed as the prime example for natural selection and thus one of the main accepted drivers for change according to Darwinian evolution in action. ‘Industrial melanism’ was the catchphrase to highlight the increase in the number of black versus speckled moths due to rising pollution in areas with developing heavy industries. Despite various criticisms from the evolutionary camp that it does not present such a well-understood example of natural selection in action after all,2 this ‘paradigm’ still experiences resurrection3 to underline its apparent unfaltering attractiveness.

Figure 1. Homing in on DNA–regulatory signalling and DNA modification (molecular genetic engineering) require an enormous and intricate amount of distinct operations. Proteins, the cellular workhorses, accomplish this masterpiece through an amazing sequence of work. The schematic shows an example of seven DNA segments (DNA double helices are arranged vertically) being worked on by proteins (represented as ribbon models within their transparent molecular surfaces) during specific tasks. These include e.g. (1) DNA transposition (movement of DNA segments, so-called transposable elements), (2) prevention and (6) activation of transcription (for protein production), (3) nucleotide flipping during DNA repair, (4) relaxing DNA during winding and unwinding, (5) transfer of a methyl group to DNA (adjustment without changing the original DNA, an example of epigenetic DNA modification) and (7) viral DNA insertion into the host genome (retroviral DNA integration).
Figure produced using PyMOL (www.pymol.org), using protein data bank entries 1muh, 1fdq, 1t39, 1a36, 3pt6, 1o4x, and 3os1.

Still looking at the front cover, a second feature stares at you. The ‘e’ of evolution is set apart in red colour from the rest of the word, which is printed in white on a black background (and with just the ‘i’ written in italics). This pattern is to be understood as a play on terminology of the Digital Age and the inventiveness of funding schemes using the ‘e’ as a placeholder for ‘electronic’ such as in e-learning, e-publishing or e-science. It is to highlight evolution as a subject matter that has come of age, which needs to embrace high performance computational and network resources as well as data handling and storage given the immense information (‘I’) content accumulated from structural and functional genomics as well as from computational systems biology since the beginning of the new century.

A novel thesis

The cover page sets the scene deftly for a “fresh look at the basics of evolution in the new century” as announced in the Introduction. Shapiro says that “we currently see only the tip of the iceberg” in view of our understanding of how such an incredible diversity of existing life arose. Despite this drawback, he introduces the stimulating idea of innovation through ‘natural genetic engineering’. The latter is regarded as the driving force for generating genomic variation once basic/simple biological organisms have acquired their directives to evolve. According to the author’s well-founded studies and investigations integrating the modern genomic concepts, the “basic fact of life” is that mutations, i.e. genetic variations do not arise by chance. Consequently natural selection can no longer be the primary shaping force of evolution. Are we witnessing a paradigm shift in evolution according to Shapiro stating, “Living cells do not operate blindly”? In other words the colourful and dazzling parade of disguise of the moth’s wings has been the result of a feedback mechanism that developed over time and eventually engraved in its genetic makeup in order to scare possible predators. However, so far the current understanding of evolution is still very much rooted in tradition—as is every so often voiced in influential journals.4

Supportive of the notion of ‘natural genetic engineering’ is the more than 50 years of built-up evidence for the need of an overhaul of evolutionary mechanisms such as the random nature of mutations, natural selection, and gradual change. For this, one may also consider the experiments by Waddington as seminal.5 The effective capabilities of cellular systems to restructure the genome by an abundance of masterstrokes (DNA insertion elements, DNA-based transposition mechanisms, mobile elements and RNA-based mutagenesis processes, to name only a few) reflect a ‘fluid genome’ prone to countless rewriting events through sensing of the environment.

Shapiro introduces the apt analogy from computing that the genome is not like a read-only but like a read-write memory, which can be formatted in numerous ways to suit the organism. Most interestingly this read-write concept is not only restricted to the small proportion of DNA (~2%) encoding proteins, i.e. the real workhorses of each organism (see figure 1), but also to significant amounts of DNA that had been written off as irrelevant (‘junk’ DNA). In this regard it is fitting to mention the very recent ENCODE project, which reported, after analyzing the functional elements of the human genome, that most of the so-called ‘junk’ is in fact very important.6 For example, it is vital for delicate and dynamic regulatory and transcriptional functions. Of further interest is also a survey of the human microbiome project7 recently revealing an immense diversity of microbes that live on and in our bodies with implications not only for medicine and public health but also for evolutionary processes. For instance theses microbes contribute to tutoring and education as well as protection of the developing human host. Yet another interconnected research area to evolution that only just glanced at the tip of the iceberg.

A well-known dilemma

Even if there is something like a cell’s own life, the primary crux of the matter is that the author doesn’t let us know the prerequisite(s) for his thesis. That is, an understanding of how the basic principles of life in a cell are defined and acquired so that at a specific stage the workings of evolutionary information processing, the ‘natural genetic engineering’, can take over. It is the latter that forms the core of the book and is described in a truly impressive, concise, and scientifically substantial manner. This will most likely be overwhelming and intense for someone who has neither a degree in modern biology nor is a scientist with interest in molecular/ cellular biology or genetics. It remains debatable whether a layperson will get something out of this technical account. This is aggravated by the fact that a further 100 pages (in addition to the above mentioned 147 pages) contain an index, a glossary comprising 25 pages with basic terms (such as gamete, homeodomain, and retrovirus), and 1,162 references, which the enthusiast can upscale by an extensive and unique on-line referencing system. Furthermore, there are no figures, schematics or diagrams to help readers understand the main points! This seems out of place, considering visual aids would be very well suited to help illustrate the numerous and intricate ways in which DNA sequences can be modified.

A reasoned layout

In the four parts, Shapiro progressively builds up a mechanistic picture of “the capacity of living organisms to alter their own heredity” from cellular to multicellular life-forms and then going beyond Darwin.

In part I, we learn in detail about molecular descriptions of how bacteria, yeast, and mammalian cells acquire knowledge through their sensory systems. These are well elucidated by specific examples, such as the sophisticated cellular regulations of the bacterial sugar metabolism (one of the author’s expert areas) and the complex cellular feats in identifying and correcting DNA defects (DNA damage repair).

Implications for the ‘Central Dogma of Molecular Biology’ formulated by Francis Crick in 19588 are revisited in the light of 21st century’s knowledge. This ‘dogma’ states that there is a directional flow of sequence information from nucleic acids (DNA/RNA) to protein. It is clear that amino acid sequences of proteins cannot be reverse-read into nucleic acid sequences. Also, inverted ribosome action is unworkable due to the redundancy of the genetic code. So one has to question Shapiro’s point of criticism. He puts the dogma’s flow of information on a level with non-adjustability of DNA information towards external stimuli, reaching the usually understood view of the genome as a read-only memory.

Mechanisms of genetic change are central to part II. Shapiro tries to underline the aforementioned comparison of the genome being a read-write storage memory that is part of the normal life cycle.

One may wonder, however, about the evolutionary potential of some of the examples presented. The adaptive immune system is indeed a textbook example for ‘natural genetic engineering’, yet there is a difference between somatic and germ cells. The adaptive immune system is associated with the former cell type, which means that genetic modifications or mutations will not be passed on to offspring, whereas there is the potential for germ cells to do so.

In contrast, part III provides higher-level discoveries in complex organisms, such as symbiotic cell fusion, horizontal DNA transfer, and the nature of protein evolution in the form of modular building blocks. These building blocks are known as (protein-) domains and represent elementary components—like parts in a construction kit—which are assembled depending on the desired overall function. They further delineate the remarkable manufacturing properties of cells and cellular constituents.

The data from molecular genetics and genome sequencing appear in favour of abrupt and saltational genomic changes “at key moments in evolution”. The last part formulates a new and radical conceptual basis for evolutionary research after reviewing and summarizing the principal directions of gathered evidence for the postulated idea of sudden, non-Darwinian ‘natural genetic engineering’. This contrasts with traditional neo-Darwinian, a slow, “selection-biased random walk through the limitless space of possible DNA configurations”. According to the author, the new concept will attract and include physical and computational scientists not being biased by the formal education in the life sciences.

Concluding remarks

Evolution—A view from the 21st century presents a modern and different view on evolution based on current genetic analyses. It is certainly of interest for scientists with aspirations in the area of modern molecular biology and genetics as well as intra-and intercellular signalling and regulation. Coincidentally it is also appealing to note the formation of two camps in evolutionary biology divided over the question of the importance of the ‘creative’ concept of natural selection in transforming organisms. Even though the title appears a bit presumptuous, it is clear that the remaining decades of this century will surely bring many more amazing findings. Some will be from serendipitous observations;9 others will result from well planned investigations such as those by large consortia.6,7 In addition emerging natural genetic engineering tools will be exploited for studying and reshaping genomes of many species.10 Not surprisingly the author highlights that there will be exciting and challenging years ahead. Yet new concepts will come and go.

The book tries to clarify that the cell is its own engineer—that there is no need for an external agent—i.e. that genomic change, remodelling, formatting, however named, represents an intrinsic vital cellular characteristic. Still what is evoking the engineering capacity, to give rise to new properties? Where does the necessary training come from? And when does a cell graduate as an engineer? Are these the key moments of evolution? According to Shapiro, random natural selection has nothing to do with it.

Remarkably, the Introduction informs about Rudolf Virchow, a contemporary of Darwin and one of the big figures in pathology. He coined the phrase omnis cellulae cellula (every cell originates from another cell). Obviously Virchow also doubted Darwin’s theory of evolution. Yet Shapiro neither accepts Darwinian selection nor a supernatural force but is in favour of a ‘third way’ (the latter is not spelled out in the book but in subsequent exchanges with ID proponents1). Startling but at the same time still encouraging, therefore, is the phrase from his book, “It requires great faith to believe that a process of random, accidental genome change could serve this function.” Thus there is still hope since great faith can accomplish much.

However, sitting on the fence is of little avail either.

“I know your works, that you are neither cold nor hot. I could wish you were cold or hot. So then, because you are lukewarm, and neither cold nor hot, I will vomit you out of My mouth” (Revelation 3:15–16, NKJV).


  1. See e.g. www.discovery.org/scripts/mt/mt-search.cgi?search=%22A+View+from+the+21st+Century%22&IncludeBlogs=2&limit=20, Accessed 23 November 2012. Return to text.
  2. See Coyne, J.A., Evolution under pressure—a look at the controversy about industrial melanism in the peppered moth, Nature 418:19–20, 2002. Return to text.
  3. Cook, L.M. et al., Selective bird predation on the peppered moth: the last experiment of Michael Majerus, Biol. Lett. 8:609–612, 2012. Return to text.
  4. E.g. Burdett, A.N., Evolution’s misleading language, Science 338:741, 2012. Return to text.
  5. E.g. Waddington, C.H., Canalization of development and genetic assimilation of acquired characteristics, Nature 183:1654–1655, 1959. Return to text.
  6. The Encyclopedia of DNA Elements (ENCODE) project, Nature 489, 6 September 2012. Return to text.
  7. Relman, D.A., Learning about who we are, Nature 486:194–195, 2012. Return to text.
  8. Crick, F.H.C., On protein synthesis: biological replication of macromolecules, Symp. Soc. Exp. Biol. 12:138–163, 1958. Return to text.
  9. Shapiro, J.A., Letting Escherichia coli teach me about genome engineering. Genetics 183:1205–1214, 2009. Return to text.
  10. Pennisi, E., The tale of the TALEs, Science 338:1408–1411, 2012. Return to text.

Readers’ comments

Damian P.
Shaun Doyle, Even though some cells have " an 'in-built' capacity for 'rewriting its own base code'." there is nothing to suggest they do do so intelligently or with deliberate intent to achieve some particular goal. It amount to nothing more than stress activated random tinkering. It is nothing at all like "a directed rewrite".

Shaun Doyle responds: If you think this 'natural genetic engineering' is no more than just stress activated random tinkering, then your disagreement is not just with me, but also with Shapiro. This is the point on which I (and the reviewer) was agreeing with Shapiro, though I (and the reviewer; not his references to ID responses to Shapiro) am convinced that he overestimates the capacity of this 'algorithmic' dynamic in the genome to produce radical structural novelty. Consider what Shapiro said in response to orthodox neo-Darwinist Jerry Coyne:
"... Jerry Coyne, who say[s] that a lot of this stuff [natural genetic engineering] is irrelevant and can all be explained by, as he puts it, 'garden-variety natural selection'. I think the responsiveness, the triggering, the specificity, the capacities that cells have for restructuring their genomes, are things which traditionally have been treated as epiphenomena in evolutionary discussions. I think now that we know about them, and now that the genome data shows us how important they have been... I think they have to come to the center of the discussion -- and I would pose the question: is natural genetic engineering more important for new features appearing in evolution than natural selection?"

"can such a notion apply to the genome in an infinitely plastic way that evolution requires?"
Evolution does not require this. IDK where you get this notion. Every organism we observe today are mutant offspring of a long line of mutants.

SD: That's precisely my point. Evolution is endless indeterministic co-option—it obviously can only work with what's present (hence "co-option"), but there's no reason to think that what's stable today will be stable 500,000 years from now (hence "indeterministic"), and there's no limit to what such indeterministic co-option could produce given enough time (hence "endless" or "infinite"). In other words, for evolution any given part of the genome is only ever metastable—no feature of the genome is ever so stable as to be changeable only by intelligent agency. However, we can give evolutionary 'pushes' to genomic systems beyond the capacity of nature (e.g. artificial selection), and yet we always run into limits to what we can do. Why? Metastability seems insufficient to explain such universal selectional 'stonewalling'. Moreover, we observe genomic systems that are conserved beyond what any evolutionist expected—e.g. the genetic switch network that governs metazoan ontogeny is so universally conserved that genetic switches in mice and fruit flies can be swapped between organisms and they still work! Evolutionists did not predict such conservation between such disparate organisms. What's worse is the lack of clues evolutionists have about how that body plan arose in the first place—it looks like it just popped up out of nowhere in a geological instant! If evolution can explain such disparate outcomes without the need for a detailed mechanistic account of how, then evolution becomes nothing more than empty naturalistic storytelling.

"Maintenance is one of the main problems for evolution"
Natural selection weeds out the failures.
SD: I agree! But that's all natural selection does—which is why natural selection is not the mechanism for generating novelty evolution requires. But the constant need for genomic maintenance also constrains the type of novelty that random and directed mutations, and even epigenetic changes, can generate.

Ferdinand III S., physical self-replicating RNA is not software which runs on an extremely deterministic system which was design specifically to only be able to run rational code (like computers and software).

SD: We have discussed self-replicating RNA before and why it's no help for abiogenesis.

"Lab-proof that cells by themselves can originate [somehow, DNA, RNA, ATP, amino acids, all at the same time?"
No one in origin of life research thinks it starts with a whole complete cell. Cells of today have evolved from much simpler precursors - self-replicating RNA most likely.

SD: Where's the proof? These 'simpler precursors' (like acellular life or protocells) have to exist beyond the minds of the naturalists who dream them up to gain any scientific traction.

Robert B.,"epigenetic effects shout CREATOR!"
Don't be fooled. Epigenes are as subject to mutation and selection as nuclear DNA.

SD: And neo-Darwinism is limited in what it can achieve regardless of whether we're considering genetics or epigenetics.

"I'm still holding out hope"
I would expect nothing less.

SD: I have appended a response that addresses this comment.
Robert B.
Shapiro's book may have value if it documents extensive epigenetic action within the genomes of many living things. To the open minded, epigenetic effects shout CREATOR!

BTW, I'm still holding out hope for an epigenetic explanation for Jacob's Ringstraked sheep; that the environment that a ewe sees when she copulates can influence the camouflage of her offspring.
Shaun Doyle
I would suggest that an epigenetic explanation is unlikely; it's more likely in the light of Genesis 31:10-13 that God stepped in and 'evened the odds' personally because Laban was cheating Jacob. In other words, the sticks did not produce the the result; God performed a miracle for Jacob. Here is a helpful (offsite) article on the issue: Does Genesis teach odd genetics?
Ferdinand III S.
"The book tries to clarify that the cell is its own engineer—that there is no need for an external agent—i.e. that genomic change, remodelling, formatting, however named, represents an intrinsic vital cellular characteristic."

In software engineering we know that code, read/write functions and changes to functional software and code - such as DNA or any aspect of genomic information - cannot and never has happened, by 'magic' or some unknown process of 'self-generation'.

What Shapiro is arguing for is not science, but some form of metaphysics based around an unknown 'force' which causes software to be coded by itself. What proof does he have ?

His claim is impossible. To state this he must offer at least Lab-proof that cells by themselves can originate [somehow, DNA, RNA, ATP, amino acids, all at the same time?]; and then progressively change the complexity of the coded material, which allows them to mutate or change into more complicated structures; with the process repeating [why, for what reason?] to achieve a new form [which form and why?].

I have never seen proof in the real world of software or code being a part of such a dialectical-naturalist process. If genomic code is software, which I believe it is, this means that his entire book is a waste of time.

I doubt that Shapiro - a metaphysician posing as a scientist - will assuage my doubts with his rhetoric and hand-waving by offering real proof, observations and experiments that I can replicated and reproduce.
Larry C.
Engineering without an engineer is a very straightforward, elegant, and simple natural process. The process is so powerfully and easily comprehended that creationists are forced to accept the bedrock underpinnings of evolution.

An underpinning of evolution is that mutations occur in an competitive population. Some mutations are vastly harmful and organisms with these mutations die or live with a disadvantage. Occasionally some mutations are vastly helpful these creatures with these mutations live with an advantage within a competitive environment. The process is very systematic and given time species will branch into variations and new species will be born after a chain of mutations.

Oddly, creationist can blatantly ignore basic foundations such as age of the universe. However even inflicted with self-imposed blindness they must accept foundations of evolution. They simply refuse to accept that evolution can produce new species. There is no logical reason for this limitation. If religion is given for limitation then god must be proven.

In my opinion evolution is a very romantic process. All living creatures are related. We are related to the flying sparrow and to the jumping grasshopper...we are even related to the stars. No divisive religion is needed.
Shaun Doyle
What you describe is neo-Darwinism, which is precisely what Shapiro thinks is inadequate for understanding how evolution works. For Shapiro, it's rather that the cell has an 'in-built' capacity for 'rewriting its own base code'. In some respects, we can agree! It wouldn't surprise us to find algorithm-like features operating within the genome that facilitate a directed rewrite of certain portions of the base code to allow for a measure of adaptability to different circumstances. But can such a notion apply to the genome in an infinitely plastic way that evolution requires? The problem is that for such algorithms to operate, the programming structure of the algorithms must remain stable, which requires maintenance of the programs. Maintenance is one of the main problems for evolution—the genome has some capacity for variation, but if there is no core stability to the genome, then the organism dies. The very purpose of maintenance is to limit the changes that take place to preserve the integrity of the system.
Damian P.
It seems this article (and Shapiro) are not advocating creationism, rather an alternate process to explain the observed change over time. Is this now acceptable simply because the process seems somewhat more likely to require divine intervention. It is just as destructive of Genesis as Darwin's original book.

Comments are automatically closed 14 days after publication.